Salmonella Serovar Diversity in Broilers: A CRISPR-SeroSeq Study
Salmonella remains one of the most significant foodborne pathogens associated with poultry production worldwide. In broiler systems, the diversity of Salmonella serovars presents a major challenge for surveillance, control strategies, and public health protection. Different serovars vary in their virulence, antimicrobial resistance patterns, and persistence within poultry farms. Understanding the distribution and dynamics of these serovars in broiler populations is therefore essential for reducing contamination along the food chain and preventing outbreaks in humans. Traditional serotyping methods, while widely used, can be labor-intensive and time-consuming. The emergence of CRISPR-based technologies has opened new possibilities for high-resolution pathogen typing. CRISPR-SeroSeq integrates clustered regularly interspaced short palindromic repeats (CRISPR) profiling with next-generation sequencing to accurately identify and differentiate Salmonella serovars. Because CRISPR loc...
